Latest paper now on – line

The latest paper is now available on line via Biochemistry. Published by the American Chemical Society, we report a potentially novel bacterial resistance mechanism for the antimicrobial peptide Maximin H5 from Bombina maxima. 

Host defense peptides show great potential for development as new antimicrobial agents however, a small number of resistance mechanisms are now being identified.  In this case the peptide bound anionic and zwitterionic membranes with low affinity whilst showing a strong ability to lyse (>55%) and penetrate (π > 6.0 mN m–1) these membranes. However, the peptide bound Escherichia coli and 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE) membranes with higher affinity and showed a very low ability for bilayer lysis (<8%) and partitioning (π > 1.0 mN m–1). Increasing levels of membrane DMPE correlated with enhanced binding by the peptide (R2 = 0.96) but inversely correlated with its lytic ability (R2 = 0.98). Taken with molecular dynamic simulations, these results suggest that maximin H5  forms multiple hydrogen bonds with phosphate and ammonium groups within PE head-groups, which in concert with charge–charge interactions anchor the peptide to the surface of E. coli membranes, inhibiting its membranolytic action.

Dennison et al  http://pubs.acs.org/doi/abs/10.1021/bi400719j

bi-2013-00719j_0006

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About Professor David Phoenix

David read Biochemistry and obtained his Bachelor of Science degree and doctorate from the University of Liverpool before obtaining a Bachelor of Arts degree from the Open University which focused on mathematics followed by a Masters in Education and a Masters in Business Administration. He is a Fellow of the Society of Biology, (FSB), the Royal Society of Chemistry (FRSC) and the Institute of Mathematics and Its Applications (FIMA). In addition his contribution to the field of medicine was recognized by the award of a Fellowship from the Royal Society of Medicine. His leadership in higher education was rewarded by election to Senior Fellowship of the Higher Education Academy. He was made an Officer of the Order of the British Empire in 2010 and was awarded Academician status by the Academy of Social Sciences in 2012.He was awarded a DSc by Liverpool University in 2009.

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